The Rate of Hydrolysis of Sucrose Essay -- Biology Science Experiments

The Rate of Hydrolysis of SucroseHypothesis - The digestion of sucrose into glucose and fructose iscarried out by the presence of the enzyme Sucrase. The activity of theenzyme depart be greatest at its optimum temperature since at thistemperature the enzymes tertiary structure pull up stakes be correct to plythe formation of the substrate-enzyme complex quickly and efficiently.This should be at 37C (body temperature), which is the temperature ofthe ileum where Sucrase is secreted and naturally functions.The first thing to say about enzymes is that they ar proteins andthey argon found in all types of organisms from humans to viruses. Theyfunction in the body as catalysts. In other words they speed up therate of chemical actions in the body. Enzymes in reality acceleratechemical reactions by a factor of about 1 million. Enzymes are organiccatalysts. Without enzymes the metabolism of an organism would be tooslow for the organism to survive. The word enzyme actually means inyeast as they were first discovered in these micro-organisms. Enzymesare found inside cells (intracellular) and outside cells (extracellular). Intracellular enzymes are for example, those that breakmetabolism while extra cellular enzymes are for example, gut digestivejuices and the enzymes secreted by bacteria, which digest their foodoutside the body then resorb the products. Enzymes work onparticular organic chemicals, these are called substrates. So ineffect food is the substrate of digestive enzymes. Each enzyme isspecific to matchless or a group of particular substrates. The majority ofthe reactions that occur in living organisms are enzyme-controlled.Without enzymes toxins would soon build up and the supply ofrespiratory substrate would decrease. Enzymes are proteins and thushave a specific shape. They are therefore specific in the reactionsthat they catalyse - one enzyme will react with blood corpuscles of onesubstrate. The site of the reaction occurs in an area on the comeof th e protein called the active site. Since the active site for allmolecules of one enzyme will be made up of the same arrangement ofamino acids, it has a highly specific shape. Generally, there is onlyone active site on each enzyme molecule and only one type of substratemolecule will control into it. Lock and key hypothesis description of thespecificity of t... ... advantageously as this the final colour was defined.Results my results from then on were much more(prenominal) reliable and theaverage was easier to plot on a graph muster my expected curve. Thefirst colour remove was a loss of the footing pink colour and the finalcolour transport is a measured blue.Throughout all these preliminary experiments and the final one all laboratory safety precautions will be observed. To check thereliability of my results I will take a mean average of all 3experiments I finally did. Using the mean of all the experiments Ithen will work out the standard deviation of the primary colour cha ngeand the final colour change.On the graphs the X axis is numbered 1-6, this corresponds to theSucrase minginess 0.0%-2.5%.Below is the preliminary experiment graph for primary colour change at0-5% solution.The line slopes down as the reaction time decreases in relation to theconcentration. This was the graph of the preliminary experiment aftermaking the changes imputable to evidence from preliminary experiment 1 and2.Prediction I expect that the higher the concentration the faster thereaction due to induced fit and lock and key hypothesis.